Toxin Testing




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Toxin Testing

Testing and handling of many bacterial toxins requires sophisticated laboratory quality controls, including CDC / APHIS Select Agent Accreditation. Few labs posses the level of expertise and have garnered the necessary credentials for Select Agent approval for toxin studies.

Currently Deibel Labs have sought the elite CDC Select Agent Approval: Deibel Labs, Inc (Madison):

  • Deibel Labs of Wisconsin, based in Madison, performs routine testing for the C.botulinum toxin, via in vivo mouse assays and cultural determinations.

Clostridium botulinum is one of two know pathogenic species of clostridia genus, the other is C.perfringens – the later is one of only a few food borne bacteria capable of both food spoilage as well as a known food borne pathogen. Although there have been the recent emergence of ELISA systems for the detection of the C.botulinum toxin, the best method commonly recognized by FDA, industry scientists and researchers is the in vivo mouse assay, due to the superior level of sensitivity seen when compared to current rapid methods.

Although the incidence of C.botulinum toxin clinical cases is quite rare, the affects of exposure to the toxin can be devastating. C.botulinum forms a pre-formed toxin, and injestion of this toxin has a paralytic affect on the body’s nerves system. In severe cases, the so called “bot toxin” destroys the nervous tissue of the body diaphragm, resulting in death due to asphyxia.

Bacillus cereusereus Toxins (Emetic and Diahhreal):
From a clinical or research perspective, this is a very interesting organism. Oddly, little is understood about its potential for food safety concerns in the Industry. The organism is ubiquitous in nature and can be readily found in almost any food type and plant environment, especially those from raw agricultural products including raw milks, rice, grains, soy, and vegetables.

Capable strains can produce one or both of two different toxins and their modes of intoxication are quite different. What is common about both types is that they may be produced before there is visible spoilage of the product.

B.cereus as a food bourne agent has been involved in outbreaks in foods with high carbohydrate concentrations, such as rice and grains, but also in milk and other dairy products, chicken, etc.

Other Toxin studies conducted at Deibel:

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